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growth factor β1  (MedChemExpress)
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A, B Relative expression levels of EMT markers VIM and CDH2 across TNM stages (T1–T4) and clinical stages (Stage I–IV) in bladder cancer (BCa) from the TCGA dataset. C–E IHC staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC versus NMIBC tissues. F–H IF staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC compared to NMIBC tissues. I–K Kaplan-Meier survival curves showing that the expression of CDH1, VIM, and CDH2 are associated with overall survival (OS) in bladder cancer patients. Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant ( p > 0.05).
Growth Factor β1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lithium phenyl 2 4 6 tri methylbenzoylphosphinate lap  (Thermo Fisher)
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A, B Relative expression levels of EMT markers VIM and CDH2 across TNM stages (T1–T4) and clinical stages (Stage I–IV) in bladder cancer (BCa) from the TCGA dataset. C–E IHC staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC versus NMIBC tissues. F–H IF staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC compared to NMIBC tissues. I–K Kaplan-Meier survival curves showing that the expression of CDH1, VIM, and CDH2 are associated with overall survival (OS) in bladder cancer patients. Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant ( p > 0.05).
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A, B Relative expression levels of EMT markers VIM and CDH2 across TNM stages (T1–T4) and clinical stages (Stage I–IV) in bladder cancer (BCa) from the TCGA dataset. C–E IHC staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC versus NMIBC tissues. F–H IF staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC compared to NMIBC tissues. I–K Kaplan-Meier survival curves showing that the expression of CDH1, VIM, and CDH2 are associated with overall survival (OS) in bladder cancer patients. Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant ( p > 0.05).
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A, B Relative expression levels of EMT markers VIM and CDH2 across TNM stages (T1–T4) and clinical stages (Stage I–IV) in bladder cancer (BCa) from the TCGA dataset. C–E IHC staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC versus NMIBC tissues. F–H IF staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC compared to NMIBC tissues. I–K Kaplan-Meier survival curves showing that the expression of CDH1, VIM, and CDH2 are associated with overall survival (OS) in bladder cancer patients. Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant ( p > 0.05).
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A, B Relative expression levels of EMT markers VIM and CDH2 across TNM stages (T1–T4) and clinical stages (Stage I–IV) in bladder cancer (BCa) from the TCGA dataset. C–E IHC staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC versus NMIBC tissues. F–H IF staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC compared to NMIBC tissues. I–K Kaplan-Meier survival curves showing that the expression of CDH1, VIM, and CDH2 are associated with overall survival (OS) in bladder cancer patients. Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant ( p > 0.05).
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A, B Relative expression levels of EMT markers VIM and CDH2 across TNM stages (T1–T4) and clinical stages (Stage I–IV) in bladder cancer (BCa) from the TCGA dataset. C–E IHC staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC versus NMIBC tissues. F–H IF staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC compared to NMIBC tissues. I–K Kaplan-Meier survival curves showing that the expression of CDH1, VIM, and CDH2 are associated with overall survival (OS) in bladder cancer patients. Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant ( p > 0.05).
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A, B Relative expression levels of EMT markers VIM and CDH2 across TNM stages (T1–T4) and clinical stages (Stage I–IV) in bladder cancer (BCa) from the TCGA dataset. C–E IHC staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC versus NMIBC tissues. F–H IF staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC compared to NMIBC tissues. I–K Kaplan-Meier survival curves showing that the expression of CDH1, VIM, and CDH2 are associated with overall survival (OS) in bladder cancer patients. Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant ( p > 0.05).
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Image Search Results


A, B Relative expression levels of EMT markers VIM and CDH2 across TNM stages (T1–T4) and clinical stages (Stage I–IV) in bladder cancer (BCa) from the TCGA dataset. C–E IHC staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC versus NMIBC tissues. F–H IF staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC compared to NMIBC tissues. I–K Kaplan-Meier survival curves showing that the expression of CDH1, VIM, and CDH2 are associated with overall survival (OS) in bladder cancer patients. Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant ( p > 0.05).

Journal: Cell Death & Disease

Article Title: Targeting the SMAD3/CISD2 axis suppresses bladder cancer progression by promoting ferroptosis in mesenchymal-like bladder cancer cells

doi: 10.1038/s41419-025-08339-9

Figure Lengend Snippet: A, B Relative expression levels of EMT markers VIM and CDH2 across TNM stages (T1–T4) and clinical stages (Stage I–IV) in bladder cancer (BCa) from the TCGA dataset. C–E IHC staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC versus NMIBC tissues. F–H IF staining of EMT markers E-cadherin, Vimentin, and N-cadherin in MIBC compared to NMIBC tissues. I–K Kaplan-Meier survival curves showing that the expression of CDH1, VIM, and CDH2 are associated with overall survival (OS) in bladder cancer patients. Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant ( p > 0.05).

Article Snippet: Chemical reagents used in this study included: recombinant human transforming growth factor β1 (TGF-β1, HY- P78168 ), ferroptosis inducers Erastin (HY-15763) and RSL3 (HY-100218A), ferroptosis inhibitor Ferrostatin-1 (Fer-1, HY-100579), and iron chelator Deferoxamine mesylate (DFO, HY-B0988), all purchased from MedChemExpress (MCE) with purity ≥98%.

Techniques: Expressing, Immunohistochemistry, Staining

A , B Western blot confirming TGF-β1-induced suppression of epithelial markers (E-cadherin) and upregulation of mesenchymal markers (Vimentin, N-cadherin). C CCK-8 assay demonstrating the effect of different concentrations of TGF-β1 on cell viability. D Transwell migration and invasion assays demonstrated changes in migratory and invasive abilities of mesenchymal-like bladder cancer cells. E , F Immunofluorescence staining of EMT markers (E-cadherin, Vimentin) in TGF-β1-treated cells. G , H RT-qPCR profiling of TGF-β1-modulated ferroptosis-related genes (GPX4, SLC7A11, FTH1, FACL4) in cells. I Western blot analysis of ferroptosis suppressor proteins (GPX4, SLC7A11) in TGF-β1-treated cells. J LPO assay showing elevated LPO levels in TGF-β1-exposed cells. K GSH assay revealing TGF-β1-induced GSH depletion in cells. L Schematic summary of TGF-β1-driven EMT promoting redox imbalance and ferroptosis sensitivity in bladder cancer cells. Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant ( p > 0.05).

Journal: Cell Death & Disease

Article Title: Targeting the SMAD3/CISD2 axis suppresses bladder cancer progression by promoting ferroptosis in mesenchymal-like bladder cancer cells

doi: 10.1038/s41419-025-08339-9

Figure Lengend Snippet: A , B Western blot confirming TGF-β1-induced suppression of epithelial markers (E-cadherin) and upregulation of mesenchymal markers (Vimentin, N-cadherin). C CCK-8 assay demonstrating the effect of different concentrations of TGF-β1 on cell viability. D Transwell migration and invasion assays demonstrated changes in migratory and invasive abilities of mesenchymal-like bladder cancer cells. E , F Immunofluorescence staining of EMT markers (E-cadherin, Vimentin) in TGF-β1-treated cells. G , H RT-qPCR profiling of TGF-β1-modulated ferroptosis-related genes (GPX4, SLC7A11, FTH1, FACL4) in cells. I Western blot analysis of ferroptosis suppressor proteins (GPX4, SLC7A11) in TGF-β1-treated cells. J LPO assay showing elevated LPO levels in TGF-β1-exposed cells. K GSH assay revealing TGF-β1-induced GSH depletion in cells. L Schematic summary of TGF-β1-driven EMT promoting redox imbalance and ferroptosis sensitivity in bladder cancer cells. Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant ( p > 0.05).

Article Snippet: Chemical reagents used in this study included: recombinant human transforming growth factor β1 (TGF-β1, HY- P78168 ), ferroptosis inducers Erastin (HY-15763) and RSL3 (HY-100218A), ferroptosis inhibitor Ferrostatin-1 (Fer-1, HY-100579), and iron chelator Deferoxamine mesylate (DFO, HY-B0988), all purchased from MedChemExpress (MCE) with purity ≥98%.

Techniques: Western Blot, CCK-8 Assay, Migration, Immunofluorescence, Staining, Quantitative RT-PCR, GSH Assay